Case study high throughput
From: Trey L.
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One of the most powerful applications of genome editing is the introduction of nucleotide substitutions in specific genomic sites. This can be used to mimic single-nucleotide polymorphisms SNPs or to generate stop codons that yield precise gene knockouts. However, screening hundreds of clones for a single edited nucleotide remains a challenge, especially in the absence of a corresponding phenotype. The final fluorescent signal indicating the presence of the introduced SNP can be detected using a standard plate reader, without requiring any special instrumentation. The fast and simple workflow comprises DNA extraction and PCR amplification of the genomic target site, followed by an enzymatic assay, using a structure-specific endonuclease that generates a fluorescent readout.
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Case Study: Increasing Machinery Throughput Without More Machines
High-throughput screening HTS is a method for scientific experimentation especially used in drug discovery and relevant to the fields of biology and chemistry. Through this process one can rapidly identify active compounds, antibodies, or genes that modulate a particular biomolecular pathway. The results of these experiments provide starting points for drug design and for understanding the noninteraction or role of a particular location. The key labware or testing vessel of HTS is the microtiter plate : a small container, usually disposable and made of plastic, that features a grid of small, open divots called wells. In general, microplates for HTS have either 96, , , , or wells.
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Applying High-Throughput Analysis to Biofilms
Understanding the mode of action of nanomaterials NMs aids in improving predictions and environmental risk assessment. In the present study, a high-throughput HTP microarray was used to study Enchytraeus crypticus gene expression. For the AgNPs, the EC 50 negatively affected apoptosis, which can lead to accumulation of abnormal cells and cause apical damage reproduction. Mechanisms which are known to be related to Ag toxicity and which were observed here for the various Ag forms included apoptosis regulation, cell redox homeostasis, impairment of energy production and response to DNA damage.
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